Table 1: Selection of mobile phases for separation of mixed standard, L-citrulline, and L-arginine by isocratic RP-HPLC.

Mobile phaseRatioSolution mixture (%)

0.7% H3P041000.7% H3P04 + 99.3% dH2O
0.1% H3P041000.1% H3P04 + 99.9% dH2O
0.7% H3P04 : ACN90 : 10(0.7% H3P04 + 99.3% dH2O) + 100% ACN