Development of Isocratic RP-HPLC Method for Separation and Quantification of L-Citrulline and L-Arginine in Watermelons

Table 1

Selection of mobile phases for separation of mixed standard, L-citrulline, and L-arginine by isocratic RP-HPLC.

Mobile phase	Ratio	Solution mixture (%)
0.7% H3P04	100	0.7% H3P04 + 99.3% dH2O
0.1% H3P04	100	0.1% H3P04 + 99.9% dH2O
0.7% H3P04 : ACN	90 : 10	(0.7% H3P04 + 99.3% dH2O) + 100% ACN