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International Journal of Analytical Chemistry
Volume 2018, Article ID 5673186, 5 pages
https://doi.org/10.1155/2018/5673186
Research Article

Quantitative Determination of 18-β-Glycyrrhetinic Acid in HepG2 Cell Line by High Performance Liquid Chromatography Method

1Istituto di Biochimica e Biochimica Clinica, Università Cattolica del Sacro Cuore Rome, Italy
2Istituto di Chimica del Riconoscimento Molecolare, CNR, c/o Università Cattolica del Sacro Cuore, L.go F. Vito 1, I-00168 Rome, Italy
3UOC Chimica, Biochimica e Biologia Molecolare, Dip. Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario A. Gemelli, IRCCS, Università Cattolica del Sacro Cuore Rome, Italy
4UOC Odontoiatria Generale e Ortodonzia, Dip Scienze dell’Invecchiamento, Neurologiche, Ortopediche e della Testa Collo. Fondazione Policlinico Universitario A. Gemelli, IRCCS, Università Cattolica del Sacro Cuore Rome, Italy
5Department of Neurosciences, Reproductive and Odontostomatological Sciences University of Naples “Federico II”, Italy
6I.M. Sechenov First Moscow State Medical University, Institute of Dentistry, Moscow, Russia

Correspondence should be addressed to Giuseppina Nocca; ti.ttacinu@accon.anippesuig

Received 6 July 2018; Accepted 4 November 2018; Published 13 November 2018

Academic Editor: Valentina Venuti

Copyright © 2018 Giuseppina Nocca et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A reverse phase high performance liquid chromatographic (RP-HPLC) method was developed for identification and estimation of 18-β-glycyrrhetinic acid (GA) in HepG2 cell line. The analysis was carried out using a JASCO HPLC system with a C-18 (3 μm) Supelco reversed phase column (150 x 4.7 mm) using a mobile phase of 80% CH3OH and 20% of CH3CN: tetrahydrofuran: water (10:80:10, v/v/v). The method was linear in the concentration range of 1.5–120 μg /mL (n = 5). The LOD and LOQ were determined based on standard deviation of the y-intercept and the slope of the calibration curve. The LOD and LOQ values were found to be 11.46 μg/mL and 34.72 μg/mL, respectively. The mean percentage recovery by standard addition experiments of GA is 92.4 % ± 5.2%. The intracellular GA concentration value, obtained as mean of five different determinations, was 45.8 ± 7.45 μg/mL. We have developed a HPLC-UV method for quantitative determination of GA inside cells, with advantages in the cost reduction and economy of the analytical process.