Development of RP-HPLC Method for the Simultaneous Quantitation of Levamisole and Albendazole: Application to Assay Validation

<table class="fixed-width table-group" id="tab1"><tr><td><table class="table"><colgroup><col style="width:9.73em"/><col style="width:27.00em"/></colgroup><tr><td class="thead-hr" colspan="2"><hr/></td></tr><tr><td class="align_left">Mobile phase</td><td class="align_center">Mixed Phosphate buffer (KH<sub>2</sub>PO<sub>4</sub>+K<sub>2</sub>HPO<sub>4</sub>): Acetonitrile 30:70</td></tr><tr><td class="align_left">pH</td><td class="align_center">3.5</td></tr><tr><td class="align_left">Column</td><td class="align_center">INERTSIL column, C18 (150x4.6 ID) 5<i>μ</i>m</td></tr><tr><td class="align_left">Flow rate</td><td class="align_center">1.0 ml/min</td></tr><tr><td class="align_left">Column temperature</td><td class="align_center">Room temperature (20-25°C)</td></tr><tr><td class="align_left">Sample temperature</td><td class="align_center">Room temperature (20-25°C)</td></tr><tr><td class="align_left">Wavelength</td><td class="align_center">224nm</td></tr><tr><td class="align_left">Injection volume</td><td class="align_center">20 <i>μ</i>l</td></tr><tr><td class="align_left">Run time</td><td class="align_center">10 min</td></tr><tr><td class="align_left">Retention time</td><td class="align_center">About 2.350min for Levamisole and 4.055min for Albendazole</td></tr><tr class="table-tr"><td colspan="2"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>Optimized chromatographic conditions.</div>

International Journal of Analytical Chemistry

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Table 1

Table 1: Development of RP-HPLC Method for the Simultaneous Quantitation of Levamisole and Albendazole: Application to Assay Validation 