Comprehensive Assessment of Degradation Behavior of Simvastatin by UHPLC/MS Method, Employing Experimental Design Methodology

Figure 2

3D surface plot representing the (a) Rs between impurity G and SIM, (b) tailing factor, and (c) Rs between peaks of impurities B and C, as a function of % organic modifier, pH, and flow rate. (d) Desirability plot for optimization of the selected responses where the red area corresponds to the optimum chromatographic conditions while ACN content maintained constant at 40%. Color change from blue to red represents increasing response values (minmax).

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