Simultaneous Determination of Seven Active Components in Rat Plasma by UHPLC-MS/MS and Application to a Quantitative Study after Oral Administration of Huang-Lian Jie-Du Decoction in High Fat-Induced Atherosclerosis Rats

Table 4

Extraction recovery and matrix effect of the components in rat plasma.

Analyte	Spiked Concentration; (ng/mL)	Extraction recovery; (%)	Matrix effect; (%)
Baicalin	2.1	83.78 ± 7.53	106.60 ± 4.96
	25.0	93.53 ± 9.84	104.82 ± 9.12
	2625	93.45 ± 7.15	95.12 ± 4.89
Baicalein	2.0	82.18 ± 5.85	96.88 ± 5.45
	25.0	89.45 ± 4.56	91.18 ± 14.56
	575	103.29 ± 8.59	93.13 ± 4.89
Wogonoside	2.0	89.78 ± 6.66	102.51 ± 11.29
	25.0	89.40 ± 5.56	103.75 ± 13.05
	1875	96.75 ± 12.82	101.02 ± 11.82
Wogonin	2.0	92.51 ± 4.12	96.14 ± 6.85
	25.7	80.52 ± 9.20	98.16 ± 10.85
	136.7	89.66 ± 12.13	99.02 ± 13.63
Berberine	0.25	95.35 ± 12.51	91.97 ± 9.50
	2.50	96.26 ± 14.99	105.17 ± 8.57
	25.0	91.89 ± 12.23	107.46 ± 5.36
Palmatine	0.25	91.06 ± 10.29	92.25 ± 7.42
	2.50	92.17 ± 6.98	107.22 ± 11.41
	25.0	89.80 ± 8.45	87.49 ± 9.29
Jatrorrhizine	0.25	88.91 ± 13.32	90.24 ± 9.03
	2.50	93.33 ± 12.98	98.79 ± 8.57
	25.0	87.78 ± 4.89	110.67 ± 10.97