Application of Multivariate Statistical Analysis to Simultaneous Spectrophotometric Enzymatic Determination of Glucose and Cholesterol in Serum Samples

Table 3

Optimized parameters and errors obtained by ANNs.

	Glucose	Cholesterol
Architecture	41-6-1	41-10-1
Number of iterations	20	24
Hidden layer transfer function	Tansig	Tansig
RMSETr^1 (mg/dL)	0.0241	0.0720
RMSEM^1 (mg/dL)	0.1221	0.1130
RMSET^1 (mg/dL)	0.0730	0.3809
RMSE, P^2 (mg/dL)	0.3865	0.5104
REP^2 (%)	9.66	12.76

They are the RMSE for training, monitoring, and testing for the calibration set, respectively.
²Errors obtained between nominal and estimated concentrations by ANNs for the calibration set.