The Determination of Parkinson’s Drugs in Human Urine by Applying Chemometric Methods

Table 3

Validation parameters for chemometric determination of levodopa and carbidopa using PLS and PCR methods.

Parameters	Method	Levodopa	Carbidopa
(nm)		280 nm	280 nm
Correlation Coefficient (R^2)		0.9997	0.9992
PRESS	PLS	0.004	0.002
	PCR	0.0001	0.002
RMSEC	PLS	4.21x10^−3	9.42x10^−3
	PCR	6.6x10^−4	9.42x10^−3
LOD (μg/mL)	PLS	0.052043	0.04631
	PCR	0.04631	0.140332
LOQ (μg/mL)	PLS	0.157706	0.052817
	PCR	0.140332	0.160051
Accuracy (% Recovery ± SD)	PLS	99.18 ± 0.74	99.61 ± 0.32
	PCR	99.18 ± 0.74	99.61 ± 0.32
Precision (Reproducibility)
Intraday (% Recovery ± SD); (n:6)	PLS	99.65 ± 0.95	99.97 ± 0.64
	PCR	99.96 ± 0.75	99.98 ± 0.42
Interday (% Recovery ± SD); (n:6)	PLS	99.75 ± 0.30	98.95 ± 0.82
	PCR	98.98 ± 0.41	99.89 ± 0.81