Optimization of Flavonoid Extraction in Dendrobium officinale Leaves and Their Inhibitory Effects on Tyrosinase Activity

Table 2

Path of steepest ascent experimental design and results.

Run	Factor 1	Factor 2	Response
	Number	Ethanol; (%)	Flavonoid; (mg/50mL)
null point(0)	1	70
step length(Δ)	1	1.5
1	1	70	12.94
2	2	71.5	20.26
3	3	73	22.67
4	4	74.5	28.49
5	5	76	30.27
6	6	77.5	33.81
7	7	79	31.13