Table 2: Path of steepest ascent experimental design and results.

RunFactor 1Factor 2Response
NumberEthanol 
(%)
Flavonoid 
(mg/50mL)

null point(0)170
step length(Δ)11.5
117012.94
2271.520.26
337322.67
4474.528.49
557630.27
6677.533.81
777931.13