Application of Factorial and Doehlert Designs for the Optimization of the Simultaneous Separation and Determination of Antimigraine Drugs in Pharmaceutical Formulations by RP-HPLC-UV

Figure 4

Representative chromatogram of standard active compounds. Mobile phase: ACN-sodium perchlorate buffer (10 mM) (38 : 62, v/v) at pH 3.5; column: Waters XBridge C18 (250 mm × 4.6 mm, 5 μm); detection at 220 nm; flow rate: 1 mL·min⁻¹.