Application of Factorial and Doehlert Designs for the Optimization of the Simultaneous Separation and Determination of Antimigraine Drugs in Pharmaceutical Formulations by RP-HPLC-UV

Table 2

Factorial design, experimental conditions, and experimental results.

Experiment no.	Experimental design					Experimental plan		Results
	X1	X2	X3	X4	U1	U2	U3	U4	Y1	Y2 (min)
1	−1	−1	−1	−1	25	2	Phosphate	30	3.7	40
2	1	−1	−1	−1	40	2	Phosphate	30	0.6	6.8
3	−1	1	−1	−1	25	7	Phosphate	30	4.1	80
4	1	1	−1	−1	40	7	Phosphate	30	0.8	13.5
5	−1	−1	1	−1	25	2	Perchlorate	30	6.0	32
6	1	−1	1	−1	40	2	Perchlorate	30	3.8	16
7	−1	1	1	−1	25	7	Perchlorate	30	6.1	60
8	1	1	1	−1	40	7	Perchlorate	30	4.2	34
9	−1	−1	−1	1	25	2	Phosphate	40	3.6	39
10	1	−1	−1	1	40	2	Phosphate	40	0.5	6.7
11	−1	1	−1	1	25	7	Phosphate	40	4.0	79
12	1	1	−1	1	40	7	Phosphate	40	0.6	13.5
13	−1	−1	1	1	25	2	Perchlorate	40	5.7	31
14	1	−1	1	1	40	2	Perchlorate	40	3.4	15.5
15	−1	1	1	1	25	7	Perchlorate	40	5.8	58
16	1	1	1	1	40	7	Perchlorate	40	3.8	30

Y₁: resolution R_s (Zol-Nara); Y₂: run time.