Application of Factorial and Doehlert Designs for the Optimization of the Simultaneous Separation and Determination of Antimigraine Drugs in Pharmaceutical Formulations by RP-HPLC-UV

Table 3

Doehlert’s experimental design, experimental conditions, and responses.

Experiment no.	Design of experiments		Operating conditions		Experimental conditions
	X1	X2	ACN (%)	pH	Y1	Y2 (min)
1	1.0000	0.0000	40.0	4.5	4.01	23.00
2	−1.0000	0.0000	25.0	4.5	6.05	44.00
3	0.5000	0.8660	36.3	7.0	4.20	37.00
4	−0.5000	−0.8660	28.8	2.0	5.70	29.00
5	0.5000	−0.8660	36.3	2.0	3.80	16.00
6	−0.5000	0.8660	28.8	7.0	5.80	55.00
7	0.0000	0.0000	32.5	4.5	5.04	35.50
8	0.0000	0.0000	32.5	4.5	5.01	35.10
9	0.0000	0.0000	32.5	4.5	5.06	35.40

Y₁: resolution R_s (Zol-Nara); Y₂: run time.