A Simple Spectrophotometric Method for Determination of Glyoxylic Acid in Its Synthesis Mixture

Figure 3

Optical absorption versus volume of concentrated sulfuric acid in the reagent with 0.25 mL glyoxylic acid 0.028 M, 0.6 mL tryptophan 0.016 M, and 2 mL of ferric chloride 0.025 M.