Establishment and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Tigecycline in Critically Ill Patients

Table 2

Matrix effect of TGC in various plasma matrices.

Nominal concentration (ng/ml)	Normalized plasma		Hemolyzed plasma		Hyperlipidemic plasma
	Matrix effects (%)	IS-normalized matrix effects (%)	Matrix effects (%)	IS-normalized matrix effects (%)	Matrix effects (%)	IS-normalized matrix effects (%)
150	209 ± 11.0	103 ± 6.9	209 ± 1.4	104 ± 5.6	200 ± 5.2	95 ± 3.2
750	182 ± 1.9	87 ± 5.2	193 ± 9.3	96 ± 6.0	179 ± 1.4	93 ± 3.8
3800	149 ± 1.6	95 ± 2.0	152 ± 3.4	95 ± 2.1	150 ± 1.5	98 ± 2.1
CV (%)	14.5	8.5	13.8	6.0	12.0	4.0