Determination of Crizotinib in Mouse Tissues by LC-MS/MS and Its Application to a Tissue Distribution Study

Table 4

Stability data of crizotinib in mouse liver and small intestines under various storage conditions (n = 3).

Storage conditions	Added	Liver		Small intestine
	(ng/mL)	Measured (ng/mL)	RE (%)	Measured (ng/mL)	RE (%)
Room temperature for 8 h	60	58.38 ± 7.50	−2.7	58.71 ± 8.59	−2.1
	6400	6520.6 ± 137.9	1.9	5887.3 ± 107.9	−8.0
Autosampler for 10 h (4°C)	60	62.05 ± 3.74	3.4	64.49 ± 4.06	7.5
	6400	6844.3 ± 79.3	6.9	6682.8 ± 348.0	4.4
Three freeze-thaw cycles	60	60.10 ± 3.35	0.2	64.21 ± 0.89	7.0
	6400	6978.5 ± 291.4	9.0	6833.4 ± 92.78	6.8
Freezing for 30 days (−40°C)	60	58.97 ± 7.04	−1.7	57.70 ± 1.24	−3.8
	6400	5803.0 ± 148.3	−9.3	6758.7 ± 209.8	5.6

RSD, relative standard deviation; RE, relative error; n, number of replicates.