Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA

Table 2

Assessment of truthfulness from bias for repeatability and reproducibility and from percent recovery for three DNA concentrations.

	DNA concentration (ng/μL)
	NIST SRM 2372			Sprague Dawley rats			Humans
	23.60	7.61	2.71	30.00	10.00	3.33	30.00	10.00	3.33
Sr	0.053	0.006	0.050	0.070	0.560	−0.350	−1.426	−0.360	−0.486
SR	−0.093	−0.076	−0.073	0.160	0.170	0.256	−0.113	−0.156	−0.190
% Recr	100.22	100.08	101.85	100.23	105.63	89.39	95.24	96.40	85.25
% RecR	98.55	98.99	102.60	100.51	101.65	107.52	99.62	98.43	94.24

S_r = repeatabiliy bias_. S_R = reproducibility bias. % Rec_r = repeatability recovery percentage. % Rec_R = reproducibility recovery percentage.