International Journal of Analytical Chemistry / 2020 / Article / Tab 1

Research Article

Simultaneous Quantitative Determination of Polyphenolic Compounds in Blumea balsamifera (Ai-Na-Xiang, Sembung) by High-Performance Liquid Chromatography with Photodiode Array Detector

Table 1

Linearity and sensitivity of the HPLC analysis.

No.Compound nameCalibration curveaR2Linear range (μg/mL)LODb (μg/mL)LOQc (μg/mL)

1Rutiny = 36771x + 4732.70.99970.37∼139.920.180.37
2Hyperosidey = 45866x − 252520.99990.34∼126.650.100.34
3Isoquercitriny = 45714x − 5732.60.99990.31∼117.200.100.31
43,3′,5,7-Tetrahydroxy-4′-methoxyflavanoney = 6982.1x − 196470.99992.67∼1001.400.892.67
53′,5,5′,7-Tetrahydroxyflavanoney = 7495.0x − 9796.11.00006.61∼330.471.325.29
6Quercetiny = 50230x − 225340.99991.15∼57.340.230.92
73,3′,4′,5-Tetrahydroxy-7-methoxyflavanoney = 5499.5x − 153310.99973.55∼443.520.883.55
8Chrysosplenol Cy = 39377x − 141160.99980.21∼51.550.070.21
9Diosmetiny = 1.0 E + 5x − 149061.00000.10∼36.060.030.10
10Tamarixetiny = 64551x − 125980.99970.11∼28.060.040.11
113,5,7-Trihydroxy-3′,4′-dimethoxyflavoney = 70721x − 196150.99990.11∼95.680.040.11
123,3′,5-Trihydroxy-4′,7-dimethoxyflavanoney = 7271.0x − 5822.31.00000.86∼747.940.290.86
13Blumeatiny = 7339.9x − 8361.51.00000.72∼627.090.240.72
14Rhamnetiny = 46787x − 117741.00000.20∼175.730.070.20
153′,4′,5-Trihydroxy-3,7-dimethoxyflavoney = 88162x − 111650.99960.06∼22.040.020.06
16Xanthoxyliny = 10671x − 5373.41.00000.99∼173.270.330.99
17Ombuiny = 1.9 E + 5x − 180060.99990.07∼23.660.020.05
183,5-Dihydroxy-3′,4′,7-trimethoxyflavoney = 74640x − 9102.70.99990.08∼20.180.030.08

y is the peak area and x is the concentration of compound (μg/mL). bLOD refers to the limit of detection, S/N = 2.3–3.6 : 1. cLOQ refers to the limit of quantification, S/N = 8.1–10.3 : 1.

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