Simultaneous Quantitative Determination of Polyphenolic Compounds in Blumea balsamifera (Ai-Na-Xiang, Sembung) by High-Performance Liquid Chromatography with Photodiode Array Detector
Table 1
Linearity and sensitivity of the HPLC analysis.
No.
Compound name
Calibration curvea
R2
Linear range (μg/mL)
LODb (μg/mL)
LOQc (μg/mL)
1
Rutin
y = 36771x + 4732.7
0.9997
0.37∼139.92
0.18
0.37
2
Hyperoside
y = 45866x − 25252
0.9999
0.34∼126.65
0.10
0.34
3
Isoquercitrin
y = 45714x − 5732.6
0.9999
0.31∼117.20
0.10
0.31
4
3,3′,5,7-Tetrahydroxy-4′-methoxyflavanone
y = 6982.1x − 19647
0.9999
2.67∼1001.40
0.89
2.67
5
3′,5,5′,7-Tetrahydroxyflavanone
y = 7495.0x − 9796.1
1.0000
6.61∼330.47
1.32
5.29
6
Quercetin
y = 50230x − 22534
0.9999
1.15∼57.34
0.23
0.92
7
3,3′,4′,5-Tetrahydroxy-7-methoxyflavanone
y = 5499.5x − 15331
0.9997
3.55∼443.52
0.88
3.55
8
Chrysosplenol C
y = 39377x − 14116
0.9998
0.21∼51.55
0.07
0.21
9
Diosmetin
y = 1.0 E + 5x − 14906
1.0000
0.10∼36.06
0.03
0.10
10
Tamarixetin
y = 64551x − 12598
0.9997
0.11∼28.06
0.04
0.11
11
3,5,7-Trihydroxy-3′,4′-dimethoxyflavone
y = 70721x − 19615
0.9999
0.11∼95.68
0.04
0.11
12
3,3′,5-Trihydroxy-4′,7-dimethoxyflavanone
y = 7271.0x − 5822.3
1.0000
0.86∼747.94
0.29
0.86
13
Blumeatin
y = 7339.9x − 8361.5
1.0000
0.72∼627.09
0.24
0.72
14
Rhamnetin
y = 46787x − 11774
1.0000
0.20∼175.73
0.07
0.20
15
3′,4′,5-Trihydroxy-3,7-dimethoxyflavone
y = 88162x − 11165
0.9996
0.06∼22.04
0.02
0.06
16
Xanthoxylin
y = 10671x − 5373.4
1.0000
0.99∼173.27
0.33
0.99
17
Ombuin
y = 1.9 E + 5x − 18006
0.9999
0.07∼23.66
0.02
0.05
18
3,5-Dihydroxy-3′,4′,7-trimethoxyflavone
y = 74640x − 9102.7
0.9999
0.08∼20.18
0.03
0.08
y is the peak area and x is the concentration of compound (μg/mL). bLOD refers to the limit of detection, S/N = 2.3–3.6 : 1. cLOQ refers to the limit of quantification, S/N = 8.1–10.3 : 1.
