A RP-HPLC Method for the Analysis of Neostigmine Methylsulfate and Process-Related Impurities, Forced Degradation Studies, in the Injection Formulation

Table 2

Gradient program.

Sr. no.	Time (min)	% of mobile phase A	% of mobile phase B
1	0.01	95	5
2	10.00	85	15
3	20.00	65	35
4	35.00	65	35
5	38.00	95	5
6	45.00	95	5