Neuroprotective Effects of Centella asiatica against Intracerebroventricular Colchicine-Induced Cognitive Impairment and Oxidative Stress

Table 4

Effect of Centella asiatica (CA; 150 and 300 mg/kg, PO) on colchicines-induced oxidative stress parameters in rat brain.

Treatment (mg/kg)	MDA levels nmol MDA/mg protein (% of sham)	Nitrite levels 𝜇mol/mg protein (% of sham)	Reduced glutathione nmol/mg protein (% of sham)	Catalase  𝜇mol of hydrogen peroxide decomposed/min/mg protein ( % of sham)	Superoxide dismutase Units/mg protein (% of sham)	Glutathione- S- transferase nmol of CDNB conjugated/min/mg protein (% of sham)
Sham	100 ± 10	100 ± 12	100 ± 10	100 ± 12	100 ± 14	100 ± 12
ACSF	114.28 ± 11	109.33 ± 11	103.33 ± 11	97.05 ± 10	97.52 ± 15	98.66 ± 16
COL	339.88 ± 32^a	298.39 ± 15^a	23.38 ± 10^a	17.98 ± 8^a	13.47 ± 3^a	18.67 ± 7^a
CA (150)	106.7 ± 15	101.29 ± 16	90.35 ± 17	93.9 ± 13.7	99.17 ± 17	97.64 ± 14
CA (300)	123.4 ± 28	99.44 ± 26	93.77 ± 15	98.8 ± 10.8	98.16 ± 16	98.65 ± 13
CA (150) + COL	229.81 ± 27^b	210.91 ± 8^b	50.45 ± 8^b	36.96 ± 6^b	39.05 ± 10^b	30.65 ± 8^b
CA (300) + COL	157.26 ± 16^b,c	148.98 ± 7.9^b,c	81.61 ± 7.6^b,c	69.71 ± 5^b,c	65.52 ± 5^b,c	65.05 ± 6^b,c

Values are mean ± SEM; ^a𝑃<.05 as compared to artificial cerebrospinal fluid- (ACSF-) injected group; ^b𝑃<.05 as compared to colchicine-injected group; ^c𝑃<.05 as compared to CA (150) + COL group; (repeated measures two-way ANOVA followed by Tukey’s test for multiple comparisons). Note: ACSF: artificial cerebrospinal fluid; COL: colchicine; CA: Centella asiatica.