Effects of Immunomodulatory Substances on Phagocytosis of A by Human Microglia
Figure 7
(a)–(c) Differential phenotype of human CHME3 microglia, in the populations of cells displaying (Aβ 1–42+, grey boxes) or not displaying (Aβ 1–42, white boxes) phagocytosis of Aβ 1–42, with regard to immunoreactivity for interleukin-1β (IL-1β), inducible nitric oxide synthase (iNOS), and IL-1β receptor type I (IL-1RI), following pretreatment with Protollin (0.001–1 g/mL). After 24–96 hours of exposure to Aβ 1–42, the cells were subjected to immunocytochemistry and analysed by flow-cytometry. The population of cells with immunoreactivity to the different markers within the Aβ 1–42+ cell population was compared with the corresponding population in the Aβ 1–42 cell population in each treatment group, using the Wilcoxon Matched Pairs Test. The data are shown as median ± percentiles (25%–75% and 10%–90%), . Statistical differences between the Aβ 1–42+ and Aβ 1–42 cells with regard to each marker are indicated by .
