AICD Overexpression in Neuro 2A Cells Regulates Expression of PTCH1 and TRPC5

Table 1

Primer sequences and PCR conditions of real-time PCR.

Name of the genes	PCR condition	PCR Cycle	Primer sequences (5′-3′)	Size (bp)
PTCHI	95°C→10 min	35	GAAAAATGAGCAGAACCATGG TGTCTTCCTTCTGAACCCCTG	102
	[95°C 30 sec, 50°C 30 sec, 60°C 1 min]
	72°C→10 min
TRPC5	95°C→10 min	35	TTCCAGCTCTCTTCACTGTGC AAGTCACAAGCCTCTCCCCAA	102
	[95°C 30 sec, 50°C 30 sec, 60°C 1 min]
	72°C→10 min
Beta actin	95°C→10 min	35	TCCTGTGGCATCCACGAAACT GAAGCATTTGCGGTGGACGAT	315
	[95°C 30 sec, 55°C 30 sec, 60°C 1 min]
	72°C→10 min