High Throughput ELISAs to Measure a Unique Glycan on Transferrin in Cerebrospinal Fluid: A Possible Extension toward Alzheimer's Disease Biomarker Development
Figure 1
PVL and SSA specifically detect Tf-1 and Tf-2, respectively. (a) CSF was electrophoresed, blotted, and stained with anti-Tf antibody (left panel), PVL (center panel), and SSA (right panel). (b) CSF (input) was sequentially precipitated by SSA-agarose and PVL-agarose. The bound and unbound proteins were electrophoresed and immunoblotted by anti-Tf antibody.
