Intracellular APP Domain Regulates Serine-Palmitoyl-CoA Transferase Expression and Is Affected in Alzheimer's Disease

Figure 3

SPTLC2 expression is reduced in the presence of functional AICD. (a) Mouse embryonic fibroblasts expressing an APP construct lacking the last 15 amino acids (aa) and therefore a functional AICD domain (MEF APP∆CT15) show increased SPT expression and activity compared to control fibroblasts (MEF WT). (b) MEF APP∆CT15 cells incubated with functional AICD peptide show compared to MEF APP∆CT15 cells incubated with solvent control decreased SPTLC2 expression. (c) MEF APP∆CT15 cells incubated with Aβ peptides and solvent control showed no difference in SPTLC2 expression. (d) SPTLC2 expression in Fe65 knock-down SH-SY5Y cells is increased.