729382.fig.003a
(a)
729382.fig.003b
(b)
Figure 3: Mutation of TrkB FL intracellular functional domains changes the effect on AICD-mediated luciferase activity. (a) The TrkB SHC isoform significantly decreases luciferase activity when compared to TrkB T ( ). The TrkB SHC Y515F mutant, unable to bind SHC, increases luciferase activity compared to TrkB SHC ( ) and does not significantly alter luciferase activity compared to TrkB T ( ). (b) TrkB Y515F (no SHC-binding) does not significantly alter luciferase activity compared to TrkB FL. TrkB K571M with an inactive tyrosine kinase domain significantly decreases luciferase activity compared to TrkB FL ( ); TrkB Y515F/K571M significantly decreases luciferase activity compared to TrkB FL ( ) but not to a greater extent of TrkB K571M. TrkB Y816F that does not bind PLC-γ also significantly decreases luciferase activity compared to TrkB FL ( ). 4–8 independent experiments with 6 replicates each, error bars SEM. 4–6 independent experiments with 6 replicates each, error bars SEM. Statistical analysis: Student’s t-test with Bonferroni correction.