Specific Silencing of L392V PSEN1 Mutant Allele by RNA Interference

Table 1

Sequences and MALDI-TOF MS data of oligoribonucleotides used for the preparation of siRNAs. The position of mismatch for pairing with the wild type gene is indicated in bold and underlined.

siRNA	Strand	Sequence	MW	MALDI-TOF MS
			(calculated)	m/z
P1	S:	5′-GUGGUUGGUAAAGCCUCAGTT-3′	6724.1	6768.3
	As:	3′-TTCACCAACCAUUUCGGAGUC-5′	6587.2	6592.1
P2	S:	5′-UGUGGUUGGUAAAGCCUCATT-3′	6685.1	6681.9
	As:	3′-TTACACCAACCAUUUCGGAGU-5′	6611.2	6608.2
P3	S:	5′-UUGUGGUUGGUAAAGCCUCTT-3′	6662.1	6660.3
	As:	3′-TTAACACCAACCAUUUCGGAG-5′	6634.2	6632.1
P4	S:	5′-GUUGUGGUUGGUAAAGCCUTT-3′	6702.1	6697.1
	As:	3′-TTCAACACCAACCAUUUCGGA-5′	6594.2	6592.6
P5	S:	5′-UGUUGUGGUUGGUAAAGCCTT-3′	6702.1	6699.6
	As:	3′-TTACAACACCAACCAUUUCGG-5′	6594.2	6590.5
P6	S:	5′-GUGUUGUGGUUGGUAAAGCTT-3′	6742.0	6736.5
	As:	3′-TTCACAACACCAACCAUUUCG-5′	6554.3	6552.9
P7	S:	5′-AGUGUU CUGGUUGGUAAAGTT-3′	6766.0	6764.0
	As:	3′-TTUCACAACACCAACCAUUUC-5′	6515.3	6514.1
P8	S:	5′-CAGUGUUGUGGUUGGUAAATT-3′	6726.1	6723.5
	As:	3′-TTGUCACAACACCAACCAUUU-5′	6555.3	6552.7
P9	S:	5′-ACAGUGUUGUGGUUGGUAATT-3′	6726.1	6725.1
	As:	3′-TTUGUCACAACACCAACCAUU-5′	6555.3	6553.2
P10	S:	5′-UACAGUGUUGUGGUUGGUATT-3′	6703.0	6705.5
	As:	3′-TTAUGUCACAACACCAACCAU-5′	6578.3	6578.6
P11	S:	5′-CUACAGUGUUGUGGUUGGUTT-3′	6679.0	6720.2
	As:	3′-TTGAUGUCACAACACCAACCA-5′	6617.2	6615.5
P12	S:	5′-UCUACAGUGUUGUGGUUGGTT-3′	6679.0	6679.4
	As:	3′-TTAGAUGUCACAACACCAACC-5′	6617.2	6617.1
P13	S:	5′-UUCUACAGUGUUGUGGUUGTT-3′	6640.1	6640.2
	As:	3′-TTAAGAUGUCACAACACCAAC-5′	6641.3	6664.1
P14	S:	5′-UUUCUACAGUGUUGUGGUUTT-3′	6601.1	6601.1
	As:	3′-TTAAAGAUGUCACAACACCAA-5′	6400.0	6701.1
P15	S:	5′-UUUUCUACAGUGUUGUGGUTT-3′	6601.1	6620.4
	As:	3′-TTAAAAGAUGUCACAACACCA-5′	6665.3	6686.7
P16	S:	5′-AUUUUCUACAGUGUUGUGGTT-3′	6624.1	6669.3
	As:	3′-TTUAAAAGAUGUCACAACACC-5′	6642.3	6640.1
P17	S:	5′-CAUUUUCUACAGUGUUGUGTT-3′	6584.1	6583.3
	As:	3′-TTGUAAAAGAUGUCACAACAC-5′	6682.2	6679.5
P18	S:	5′-UCAUUUUCUACAGUGUUGUTT-3′	6545.2	6583.8
	As:	3′-TTAGUAAAAGAUGUCACAACA-5′	6706.2	6705.0
P19	S:	5′-UUCAUUUUCUACAGUGUUGTT-3′	6545.2	6543.6
	As:	3′-TTAAGUAAAAGAUGUCACAAC-5′	6706.2	6704.4