Fluorescent Analysis of the Cell-Selective Alzheimer's Disease Aβ Peptide Surface Membrane Binding: Influence of Membrane Components
Figure 2
Correlation between Aβ-FAM binding and the levels of surface phosphatidylserine in different types of cells. Hippocampal neurons, PC12, and GT1-7 cells were double fluorescence labeled with Aβ-FAM and annexin V-PE. The histograms show fluorescence analysis from the three types of cells based on levels of intensity of the cell-bound annexin V-PE of each one of the subpopulations as defined in Figure 1. There is a remarkable correlation between the level of Aβ-FAM binding and the level of PtdSer. The table below shows representative results in terms of the percentage of cells (%) and the annexin V-PE Geometric-Mean of Fluorescence (GeoMF) in the gate .
