Ceramide and Related-Sphingolipid Levels Are Not Altered in Disease-Associated Brain Regions of APP^SL and APP^SL/PS1^M146L Mouse Models of Alzheimer's Disease: Relationship with the Lack of Neurodegeneration?

Figure 3

Typical HPTLC profiles of ceramides from hippocampus of 12-month-old APP^SL/PS1^M146L, PS1^M146L, and their age-matched wild-type (WT) control mice (a), and from cerebral cortex and cerebellum of 24-month-old APP^SL mice and age-matched WT control ((b) and (c), resp.). The densitometric analyses of the HPTLC plates were shown in parallel, respectively. Std: a mixture of standard NFA-ceramides and 2-HFA-ceramides. Ceramides were developed on HPTLC plates with chloroform-methanol (50 : 3) and located by spraying the plate with copper acetate 3% in H₃PO₄ 8% reagent, followed by heating. Both 2-HFA-Cer and NFA-Cer appear as two bands corresponding to long-chain ceramides (≥22C; upper band) and short-chain ceramides (≤18C; lower band). Quantitative evaluation of ceramides was performed by scanning the plate at 396 nm with the Camag TLC Scanner 3/WinCats software system.

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(b)

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