Table of Contents Author Guidelines Submit a Manuscript
International Journal of Alzheimer’s Disease
Volume 2011 (2011), Article ID 925073, 8 pages
Research Article

Spectroscopic Characterization of Intermolecular Interaction of Amyloid β Promoted on GM1 Micelles

1Graduate school of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603, Japan
2Institute for Molecular Science and Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji, Okazaki 444-8787, Japan
3Hiroshima Synchrotron Radiation Center, Hiroshima University, 2-313 Kagamiyama, Higashi-Hiroshima 739-0046, Japan
4Department of Alzheimer's Disease Research, National Center for Geriatrics and Gerontology, National Institute for Longevity Sciences, 36-3 Gengo, Morioka, Obu, Aichi 474-8522, Japan

Received 13 October 2010; Revised 30 November 2010; Accepted 3 December 2010

Academic Editor: J. Fantini

Copyright © 2011 Maho Yagi-Utsumi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

1H-15N HSQC spectra of 15N-labelled Aβ(1–40) titrated with GM1. Spectral data were obtained using 0.2 mM Aβ(1–40) proteins uniformly 15N-labelled (black) or selectively 15N-labelled at Val39 (green) or Val40 (red). Aβ/GM1 molar ratios were (A) 1 : 0, (B) 1 : 2, (C) 1 : 5, (D) 1 : 15, (E) 1 : 20 and (F) 1 : 30.

  1. Supplementary Figure