Knockdown of BACE1-AS Nonprotein-Coding Transcript Modulates Beta-Amyloid-Related Hippocampal Neurogenesis
β-Amyloid peptide accumulates with aging in Tg-19959 APP transgenic mice. The level of soluble Aβ 1–42 and Aβ 1–40 amyloid peptides in prefrontal cortex (PFC), hippocampus anterior (HPA), hippocampus posterior (HPP), striatum (ST), and cerebellum (CB) at different ages, compared to wild type controls were quantified by AlphaLISA. Both Aβ 1–42 (a) and Aβ 1–40 (b) were gradually increased as animals aged, in all regions studied. The Western blot of hippocampal samples at different ages using the 48G antibody shows an increase over time in specific aggregation of Aβ (c). Immunohistochemistry with the 4G8 antibody against Aβ at different ages shows insoluble Aβ deposits in Tg-19959 hippocampi as early as in 2-month-old animals. (d): 2 m/o, (e); 6 m/o, (f): 10 m/o, (g): 13 m/o, and (h): 26 m/o. Green: 4G8. Blue: Hoechst.
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