Association between the c. 2495 A>G ATP7B Polymorphism and Sporadic Alzheimer's Disease

Table 1

Oligonucleotides sequences.

	Primer forward for sequencing	Primer reverse for sequencing	Annealing temperature	PCR product
Exon 2a	5^'AGAGGCCGTCATCACTTATC 3′	5^'CAATGGCAATCAGAGTGGTA 3^'	57°C	255 bp
Exon 2b	5^'AGCTCCTAGGGGTTCAAAGT 3′	5^' CAAGGAAAGTTTGCAGGATT 3^’	57°C	584 bp
Exon 5	5^'TTTCACAGGCTTTCCTTGAT 3′	5^' ATTTCCATGGGAAAAGTTGA 3^'	53°C	336 bp
Exon 8	5^'CGACTGTGCACAAAGCTAGA 3′	5^'CATGGTGTTCAGAGGAAGTG 3^'	54°C	386 bp
Exon 10	5^’CAGCTGGCCTAGAACCTGAC 3′	5^'TATCCTCCTGAGGGAACAT 3^'	53°C	234 bp
Exon 14	5^'CTGTGCAGGTGTCTTGTTTC 3′	5^'TTTTCCAGACCACACAGAGA 3^'	57°C	407 bp
Exon 16	5^'TGTCCTAAAGGATGCTGTCA 3′	5^'GGAAAACAGGCCTGAAATTA 3^'	55°C	451 bp
	Primer forward for allele-specific PCR	Primer reverse for allele-specific PCR
Exon 10	5^’CAGCTGGCCTAGAACCTGACCC 3^’	5^’GAAACTTTCCCCCAGGGACCACCT 3^’; 5^’ACTTTCCCCCAGGGACCACCC 3^’	63°C	141 bp
Beta actin	5^'GTCACATCCAGGGTCCTCAC 3^'	5^’CACCTTCACCGTTCCAGTTT 3^'	65°C	350 bp