Shilajit: A Natural Phytocomplex with Potential Procognitive Activity

Table 1

Morphometric study of primary cultured rat hippocampal cells exposed to Shilajit and the Brain Up-10 formulae that contain Shilajit plus complex B vitamins (Vit B6, B9, and B12).

	Control	Shilajit**	Brain Up-10*
Neuronal cells per field	367 ± 23	345 ± 42	396 ± 16.0
Percentage of cells with neuronal processes	18.0 ± 2.1	26.0 ± 3.2**	43.0 ± 3.1**
Fraction of axon-like processes	0.22	0.29	0.41
Processes length (μm)	17.4 ± 7.2	26.0 ± 4.5**	39.6 ± 8.0**

Hippocampal cells were grown in Petri dishes in the presence of either 10 mg/mL Shilajit or the formulation of Brain Up-10 [30] plus vitamins of the B complex. In the control, cells were grown in culture medium without Shilajit or the formulation. Mean of 5 determinations (𝑛=5) (significance of differences with respect to control, **𝑃<0.001).