Microglial Amyloid-𝜷1-40 Phagocytosis Dysfunction Is Caused by High-Mobility Group Box Protein-1: Implications for the Pathological Progression of Alzheimer’s Disease

Figure 3

Effect of exogenous HMGB1 on microglial A𝛽 phagocytosis analyzed by Western blot. Rat microglia were incubated with the vehicle, HMGB1, A𝛽40, or A𝛽40 and HMGB1 for 12 h. Microglial cell lysate (a) and conditioned medium (b) were then subjected to Western blot analysis using the anti-A𝛽 antibody, and then the amounts of A𝛽40 inside (a) and outside microglia (b) were semiquantitatively measured. *𝑃<0.05, ***𝑃<0.001 versus treatment with A𝛽40 alone.

(a)

(b)