Figure 3: Effect of exogenous HMGB1 on microglial A phagocytosis analyzed by Western blot. Rat microglia were incubated with the vehicle, HMGB1, A 40, or A 40 and HMGB1 for 12 h. Microglial cell lysate (a) and conditioned medium (b) were then subjected to Western blot analysis using the anti-A antibody, and then the amounts of A 40 inside (a) and outside microglia (b) were semiquantitatively measured. * , *** versus treatment with A 40 alone.