Table of Contents
International Journal of Antibiotics
Volume 2014 (2014), Article ID 935842, 7 pages
http://dx.doi.org/10.1155/2014/935842
Research Article

The Occurrence of blaCTX-M, blaSHV, and blaTEM Genes in Extended-Spectrum β-Lactamase-Positive Strains of Klebsiella pneumoniae, Escherichia coli, and Proteus mirabilis in Poland

1Department of Microbiological Diagnostics and Infectious Immunology, Medical University of Bialystok, 15a Waszyngtona Street, 15-269 Bialystok, Poland
2Department of Anaesthesiology and Intensive Therapy, Medical University of Bialystok, 37 Szpitalna Street, 15-274 Bialystok, Poland
3Department of Microbiological Diagnostics and Infectious Immunology, University Hospital of Bialystok, 15a Waszyngtona Street, 15-269 Bialystok, Poland
4Hospital Emergency Department with Intensive Care Subdivision, University Hospital of Bialystok, 24a M. Skłodowskiej-Curie Street, 15-276 Bialystok, Poland

Received 28 October 2013; Accepted 25 December 2013; Published 6 February 2014

Academic Editor: Cheol-In Kang

Copyright © 2014 Dominika Ojdana et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Bacteria belonging to the Enterobacteriaceae family that produce extended-spectrum β-lactamase (ESBL) enzymes are important pathogens of infections. Increasing numbers of ESBL-producing bacterial strains exhibiting multidrug resistance have been observed. The aim of the study was to evaluate the prevalence of , , and genes among ESBL-producing Klebsiella pneumoniae, Escherichia coli, and Proteus mirabilis strains and to examine susceptibility to antibiotics of tested strains. In our study, thirty-six of the tested strains exhibited genes , , , and . Moreover, twelve ESBL-positive strains harbored genes , , , and , and the presence of a gene in twenty-five ESBL-positive strains was revealed. Among K. pneumoniae the multiple ESBL genotype composed of , and genes encoding particular ESBL variants was observed. Analysis of bacterial susceptibility to antibiotics revealed that, among β-lactam antibiotics, the most effective against E. coli strains was meropenem (100%), whereas K. pneumoniae were completely susceptible to ertapenem and meropenem (100%), and P. mirabilis strains were susceptible to ertapenem (91.7%). Moreover, among non-β-lactam antibiotics, gentamicin showed the highest activity to E. coli (91.7%) and ciprofloxacin the highest to K. pneumoniae (83.3%). P. mirabilis revealed the highest susceptibility to amikacin (66.7%).