Table of Contents
International Journal of Bacteriology
Volume 2013, Article ID 203432, 9 pages
http://dx.doi.org/10.1155/2013/203432
Research Article

New Medium for Pharmaceutical Grade Arthrospira

1Department of Protein Research, Genetic Engineering and Biotechnology Research Institute, (Mubarak) City for Scientific Research and Technology Applications, Universities and Research Centre District, New Borg El-Arab, P.O. Box 21934, Alexandria, Egypt
2Institut für Molekulare Mikrobiologie ünd Biotekhnologie, Westfälishen Wilhelms-Universität Münster, Corrensstraße 3, 48149 Münster, Germany

Received 29 May 2013; Revised 3 November 2013; Accepted 14 November 2013

Academic Editor: Gary Dykes

Copyright © 2013 Amro A. Amara and Alexander Steinbüchel. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study is to produce a pharmaceutical grade single cell product of Arthrospira from a mixed culture. We have designed a medium derived from a combination between George’s and Zarrouk’s media. Our new medium has the ability to inhibit different forms of cyanobacterium and microalgae except the Chlorella. The medium and the cultivation conditions have been investigated to map the points where only Arthrospira could survive. For that, a mixed culture of pure Chlorella and Arthrospira (~90 : 10) has been used to develop the best medium composition that can lead to the enrichment of the Arthrospira growth and the inhibition of the Chlorella growth. To enable better control and to study its growth, an 80 l photobioreactor has been used. We have used high saline (2xA-St) medium which has been followed by in fermentor reducing its concentration to 1.5x. The investigation proves that Chlorella has completely disappeared. A method and a new saline medium have been established using a photobioreactor for in fermentor production of single cell Arthrospira. Such method enables the production of pure pharmaceutical grade Arthrospira for medicinal and pharmaceutical applications or as a single cell protein.