Expression, Purification, and Functional Characterization of Atypical Xenocin, Its Immunity Protein, and Their Domains from Xenorhabdus nematophila
Figure 3
Neutralization of endogenous toxicity of xenocin expressed under its native promoter in the presence of mitomycin C. (a) Bacterial growth was monitored by determining the optical density at 600 nm; (■): XIM strain induced; (♦): GEM strain induced. (b) Exogenous toxicity assay using E. coli DH5 grown in M9 medium as target cells. (i) Purified recombinant xenocin-immunity domain protein complex; (ii) purified recombinant catalytic-immunity domain protein complex; (iii) purified recombinant immunity protein (42 kDa); (iv) purified recombinant hemolysin domain (32 kDa) of immunity protein; (v) buffer control.
