Research Article
Expression, Purification, and Functional Characterization of Atypical Xenocin, Its Immunity Protein, and Their Domains from Xenorhabdus nematophila
Table 1
Strains and plasmids used in this study.
| | Construct/strain | Characteristic | Source |
| | E. coli DH5α | supE44ΔlacU169 hsdR17 recA1 endA1 gyrA96 thi-1 relA1Φ80 dlacZ ΔM15 | Invitrogen | | E. coli M 15 | NaISStrSRIFSThi−Lac−Ara+Gal+MtI−F−RecA+Uvr+Lon+ | Qiagen | | E. coli BL21 (DE3) pLysS | F−ompT hsdSB() gal dcm(DE3)pLysS(CmR) | Novagen | | pGEM-T Easy | 3 kb vector for cloning PCR fragments; Ampr | Promega | | pQE30 and 31 | 3.4 kb expression vector; Ampr | Qiagen | | pET 28 (a) | 5.3 kb expression vector; kanr | Novagen | | pJC2/JC2 | pQE30 containing (xcinA) and ximB genes. | Ref. [13] | | pJC4/JC4 | pET28 containing catalytic domain and 270 bp partial ximB (N′ terminal 86 amino acids) | Ref. [13] | | pJC5/JC5 | pGEM-T Easy containing xcinA and ximB (255 bp) genes | This study | | pJC6/JC6 | pQE30 containing xcinA and ximB (255 bp) genes | This study | | pJC7/JC7 | pGEM-T Easy containing translocation domain of xcinA gene | This study | | pJC8/JC8 | pQE30 containing translocation domain of xcinA gene | This study | | pJC9/JC9 | pGEM-T Easy containing translocation-receptor domain of xcinA gene | This study | | pJC10/JC10 | pQE30 containing translocation-receptor domain of xcinA gene | This study | | pJC11/JC11 | pGEM-T Easy containing ximB gene | This study | | pJC12/JC12 | pGEM-T Easy containing hemolysin domain of ximB gene | This study | | pJC13/JC13 | pQE30 containing ximB gene | This study | | pJC14/JC14 | pQE31 containing hemolysin domain of ximB gene | This study | | pXIM/XIM | pGEM-T Easy containing xcinA (with native promoter) and ximB (255 bp) genes | This study | | pGEM/GEM | pGEM-T Easy in E. coli DH5 | This study |
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