Table of Contents
International Journal of Bacteriology
Volume 2014, Article ID 860491, 10 pages
http://dx.doi.org/10.1155/2014/860491
Research Article

Phylogenetic Framework and Biosurfactant Gene Expression Analysis of Marine Bacillus spp. of Eastern Coastal Plain of Tamil Nadu

1Microbiology Division, CSIR-Central Leather Research Institute, Adyar, Chennai, Tamil Nadu 600 020, India
2Environmental Technology Division, CSIR-Central Leather Research Institute, Adyar, Chennai, Tamil Nadu 600 020, India

Received 15 October 2013; Accepted 17 December 2013; Published 12 February 2014

Academic Editor: Rodrigo E. Mendes

Copyright © 2014 Sreethar Swaathy et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present study emphasizes the diversity assessment of marine Bacillus species with special reference to biosurfactant production, respective gene expression, and discrimination among Bacillus licheniformis and Bacillus subtilis. Among the 200 individual species of eastern coastal plain of Tamil Nadu screened, five biosurfactant producing potential bacterial species with entirely different morphology were selected. Biochemical and 16S rRNA gene sequence analysis suggested that all the said five species belong to Bacillus genera but differ in species levels. Biosurfactant of all the five species fluctuates in greater levels with respect to activity as well as to constituents but showed partial similarity to the commercially available surfactin. The expression of srf gene was realized in all of the five species. However, the sfp gene expression was observed only in three species. In conclusion, both B. licheniformis and B. subtilis demonstrate srf gene; nevertheless, sfp gene was expressed only by Bacillus subtilis.