Phylogenetic Framework and Biosurfactant Gene Expression Analysis of Marine Bacillus spp. of Eastern Coastal Plain of Tamil Nadu

Table 1

Phenotypic and biochemical characteristic of marine isolates.

Characteristics	Isolate 1	Isolate 2	Isolate 3	Isolate 4	Isolate 5
Morphology	Pink, dry, and filamentous	White, smooth, and irregular colonies	White, smooth, circular, and undulate margin	White, circular, and smooth margin	White, smooth, slimy, and entire margin
Gram stain	+	+	+	+	+
Shape	Rod	Rod	Rod	Rod	Rod
Motility	Motile	Motile	Motile	Motile	Motile
Indole	−	−	−	−	−
Methyl red	−	−	−	−	−
Voges Proskauer	+	+	+	+	+
Citrate	+	+	+	+	+
Urease	−	−	−	−	+
Nitrate	+	+	−	−	+
Oxidase	+	−	−	−	−
Catalase	+	+	+	+	+
Starch hydrolysis	+	+	+	+	+
Casein hydrolysis	+	+	+	+	+
Lipid hydrolysis	+	+	+	+	+
Gelatin hydrolysis	+	+	+	+	+
Growth at 55°C	+	+	−	−	−
Growth in NaCl
5%	+	+	+	+	+
7.5%	+	+	+	+	+

(+): positive; (−): negative.