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This article has been retracted as it was found to be substantially similar to the following previously published article: “Biodegradation Of Crude Oil Using Local Isolates,” by Aboelwafa, A. M., and Alwasify, R. S., published in Australian Journal of Basic and Applied Sciences.

View the full Retraction here.


  1. R. S. Al-Wasify and S. R. Hamed, “Bacterial biodegradation of crude oil using local isolates,” International Journal of Bacteriology, vol. 2014, Article ID 863272, 8 pages, 2014.
International Journal of Bacteriology
Volume 2014, Article ID 863272, 8 pages
Research Article

Bacterial Biodegradation of Crude Oil Using Local Isolates

1Water Pollution Research Department, National Research Centre, Dokki, Giza 12622, Egypt
2Microbial Biotechnology Department, National Research Centre, Dokki, Giza 12622, Egypt

Received 31 October 2013; Accepted 8 December 2013; Published 20 January 2014

Academic Editor: Roman R. Ganta

Copyright © 2014 Raed S. Al-Wasify and Shimaa R. Hamed. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


An experimental study was undertaken to assess the efficiency of Pseudomonas aeruginosa, Bacillus subtilis, and Acinetobacter lwoffi isolated from petroleum contaminated water and soil samples to degrade crude oil, separately and in a mixed bacterial consortium. Capillary gas chromatography was used for testing the effect of those bacterial species on the biodegradation of crude oil. Individual bacterial cultures showed less growth and degradation than did the mixed bacterial consortium. At temperature 22°C, the mixed bacterial consortium degraded a maximum of 88.5% of Egyptian crude oil after 28 days of incubation. This was followed by 77.8% by Pseudomonas aeruginosa, 76.7% by Bacillus subtilis, and 74.3% by Acinetobacter lwoffi. The results demonstrated that the selected bacterial isolates could be effective in biodegradation of oil spills individually and showed better biodegradation abilities when they are used together in mixed consortium.