Role of Burkholderia pseudomallei Sigma N2 in Amino Acids Utilization and in Regulation of Catalase E Expression at the Transcriptional Level

Table 1

Bacterial strains and plasmids used in this study.

Strain or plasmid	Genotype or relevant characteristic	Source (reference)
Bacteria strains
PP844	Wild type, clinical isolate from blood	This study
E. coli SM10 λpir	λpir (thi thr leu tonA lacY supE  recA::RP4-2-Tc::Mu Km) used for transformation of recombinant plasmid pKNOCK::rpoN2	[15]
E. coli JKD 814	rpoN::tet	[16]
Plasmids
pKNOCK-Tc	Mobilizable suicide vector carrying Tet gene	[10]
pKRpoN2	pKNOCK-Tc containing 363 bp internal segment of B. pseudomallei rpoN2 gene	This study
pBBR1MCS	Broad-host-range cloning vector, Cm	[17]
pBSDRpoN2	pBR1MCS containing full-length rpoN2 gene	This study
pCR 2.1-TOPO	Cloning vector for PCR product, Kanamycin and Ampicillin resistance	Invitrogen, California, USA
pTOPO::rpoN2	pCR 2.1-TOPO vector containing 799 bp fragment of truncated rpoN2 gene from B. pseudomallei strain 844, Kanamycin and Ampicillin resistance	This study
pTOPO::rpoN	pCR 2.1-TOPO vector containing full-length rpoN gene from E. coli, Kanamycin and Ampicillin resistance	This study