Role of Burkholderia pseudomallei Sigma N2 in Amino Acids Utilization and in Regulation of Catalase E Expression at the Transcriptional Level
Table 2
Nitrogen and amino acid utilization of B. pseudomallei wild type 844 and E. coli derivatives.
Nitrogen source
Day of growth appearance (mean ± SD)
E. coli JM 109
JKD 814 rpoN mutant
JKD 814 harboring pTOPO vector
JKD 814 harboring TOPO::rpoN
JKD 814 harboring TOPO::rpoN2
JKD 814 harboring TOPO::rpoN2
B. pseudomallei wild type 844
NH4Cl
3.7 ± 0.6
±
±
2.7 ± 0.6
2 ± 1
NG
4.7 ± 0.6
Arginine
1.3 ± 0.6
±
±
3.3 ± 0.6
3.3 ± 0.6
±
4.7 ± 0.6
Glutamine
2.7 ± 0.6
NG
NG
4.6 ± 0.6
3.7 ± 0.6
NG
1.3 ± 0.6
Glycine
2.7 ± 0.6
±
±
2.3 ± 0.6
2.7 ± 0.6
±
±
Histidine
1.3 ± 0.6
NG
NG
4 ± 1
2.3 ± 0.6
NG
1.3 ± 0.6
Lysine
3.3 ± 0.6
NG
NG
3.7 ± 0.6
4.3 ± 1.2
NG
4.7 ± 0.6
Methionine
4.7 ± 0.6
NG
NG
NG
4.7 ± 0.6
NG
±
Phenylalanine
4.7 ± 0.6
NG
±
4.7 ± 0.6
3.7 ± 0.6
±
1.3 ± 0.6
Tryptophan
1.7 ± 0.6
±
±
1.7 ± 0.6
1.7 ± 0.6
NG
2.7 ± 0.6
Valine
1.3 ± 0.6
NG
NG
NG
NG
NG
4.7 ± 0.6
Data represent geometric mean (±standard error) from three independent experiments. NG indicates the absence of growth. ± indicates the very sparing growth inspected on day 6. Significant differences in amino acid utilization compared to E. coli rpoN mutant JKD 814 (, Student’s paired -test).
