Table of Contents
International Journal of Bacteriology
Volume 2016 (2016), Article ID 7146470, 8 pages
http://dx.doi.org/10.1155/2016/7146470
Research Article

Genotyping of Mycobacterium tuberculosis Isolates from Hormozgan Province of Iran Based on 15-Locus MIRU-VNTR and Spoligotyping

1Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
3Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
4Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5Applied Microbiology Research Centre, Baqiyatallah University of Medical Sciences, Tehran, Iran
6Molecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
7Thorax Research Center, Tehran University of Medical Sciences, Tehran, Iran

Received 3 May 2016; Accepted 25 September 2016

Academic Editor: Sam R. Telford

Copyright © 2016 Samin Zamani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Considering that Hormozgan province in Iran (southern part of Iran on the Persian Gulf) is among the areas with high prevalence of MDR-MTB and attracts so many sailors and tourists, genetic diversity of MTB isolates circulating in this part of Iran was evaluated. Pattern of TB transmission was also examined. Methods and Material. A total of 38 isolates of MTB were cultured from TB patients from Hormozgan province of Iran and standard MIRU-VNTR typing and spoligotyping were applied to genotype these isolates. Drug susceptibility testing was performed using proportion method. Results. There were 28 VNTR profiles comprising 5 clusters and 23 unique isolates compared to 21 spoligotyping profiles, which contained 9 clusters and 12 unique isolates. Latin American-Mediterranean (, 23.6%) was found to be the most predominant lineage. MIRU-VNTR analysis, with an HGDI of 0.975, was more discriminating than spoligotyping, which had an HGDI of 0.955. The estimated proportion of TB cases due to recent transmission was 26.3% and 44.7% by MIRU-VNTR and spoligotyping, respectively. The rates of monodrug resistance and MDR were 15.8% and 7.9%, respectively. Two of 3 MDR strains were found to be related to MIRU-VNTR and belonged to the same spoligotyping cluster characterized with T1/SIT53 genotype. Conclusions. The high genetic diversity among MTB isolates suggests that transmission occurred from different sources to this area. Reactivation of a priori, latent MTB infection was found to contribute mainly to TB cases in this geographic region.