Changes in Cytokines of the Bone Microenvironment during Breast Cancer Metastasis
Breast cancer metastases in femoral bone. Female athymic nude mice were inoculated in the left cardiac ventricle with MDA-MB- or MDA-MB-231BRMS cells, eight per group. Animals were euthanized at day 3, 11, 19, or 27 after injection. Femurs were removed, placed in PBS, and imaged with light and fluorescence stereomicroscopy at a 40x magnification to detect GFP-labeled cancer cell metastases. Images are shown for 27 day metastases (a) of MDA-MB-231BRMS1 (top) and MDA-MB-231 (bottom). Note colony size difference between the two variants. Scale bar = 1 mm. Table (b) summarizes the incidence of detectable GFP-expressing cells for each injection group.
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