Selection of a MCF-7 Breast Cancer Cell Subpopulation with High Sensitivity to IL-1β: Characterization of and Correlation between Morphological and Molecular Changes Leading to Increased Invasiveness
Figure 3
MCF-7A3 cells showed increased chemotaxis and invasiveness than parental MCF-7pl cells. (A) Chemotaxis assay. MCF-7 and MCF-7 A3 cells were stimulated with IL-1β for 24, 48, 72 h. After stimulus, cells were transferred to Transwell chambers and allowed to migrate for 24 h in presence of the chemoattractant, CXCL12. Cells that migrated to the lower side of the filter were fixed with PFA and nuclei stained with DAPI. Fluorescent nuclei were counted in acquired digital images of 10 randomly chosen fields in each condition. (B) Box plots show the number of cells that migrated after IL-1β stimulus. (C) Invasion assay. Cells were treated in a similar way as in the chemotaxis assay, except for the use of a Matrigel layer deposited on top of the filter of the chamber and the absence of the chemoattractant CXCL12 in the culture medium. (D) Box plots show the number of cells that invaded and broke down Matrigel to cross to the lower side of the filter after IL-1β stimulus. Data in (B) and (D) are presented as median ± SD of the values obtained for each time in three independent experiments. *; **.
