Preclinical Evaluation of Oncolytic Δγ134.5 Herpes Simplex Virus Expressing Interleukin-12 for Therapy of Breast Cancer Brain Metastases
Figure 3
Replication of M002 over time. In a multistep replication assay, HSV replication was assayed in the following lines: the permissive Vero cell line (a), human breast cancer MDA-MB-361 (b), and murine mammary carcinoma lines SCK (c) and 4T1 (d). Cells were infected with HSV-1 (F) (triangles), R3659 (diamonds), and M002 (boxes) at an MOI of 0.1 PFU/cell. At multiple time points, cells and media were harvested, and viral replication was assayed by titration on Vero cell monolayers.