Comparison of Fluorescence In Situ Hybridization and Chromogenic In Situ Hybridization for Low and High Throughput HER2 Genetic Testing

Table 1

Different characteristics between the five HER2 genetic assays.

	Dako HER2 FISH		Dako HER2 IQ-FISH		Dako HER2 CISH		ZytoVision HER2 FISH		ZytoVision HER2 CISH
Gene	HER2	CEN17	HER2	CEN17	HER2	CEN17	HER2	CEN17	HER2	CEN17
Probe	DNA	PNA	DNA	PNA	DNA	PNA	DNA	DNA	DNA	DNA
Label color	TxRed	FITC	TxRed	FITC	Red	Blue	FITC	Rhodamine	Green	Red
Blocking reagent	alu-PNA		alu-PNA		alu-PNA		Repeat free		Repeat free
Visualization	Fluorescence		Fluorescence		Chromogenic		Fluorescence		Chromogenic
Hybridization reagent	Formamide		Ethylene carbonate		Formamide		Formamide		Formamide

CISH: chromogenic in situ hybridization; FISH: fluorescence in situ hybridization; PNA: peptide nucleic acids.