Phenotypic and Molecular Characterization of MCF10DCIS and SUM Breast Cancer Cell Lines

Table 3

p53 profile of the Asterand SUM cell lines.

Cell line	17p allelic loss	p53 gene sequence				p53 expression		p14ARF status
		Exon	Mutation	Amino acid change	Protein effect	Transcript size (kb)	Transcript expression	Gene	Transcript expression
SUM102PT					Wild type		nd	Deleted	−
SUM1315MO2	Loss	5	TGC>TTC	C135F	Missense	1	+	Deleted	−
SUM159PT	Loss	5	TCC>TCCTCC	S158insS	Missense	0.7	−		+
SUM149PT	Loss	7	ATG>ATA	M237I	Missense	1	+	Deleted	−
SUM225CWN	Loss	8	CTG>CCG	L265P	Missense	0.9	++	nd	nd
SUM229PE	Loss	8	CGT>TGT	R273C	Missense	0.7	nd	Deleted	−
SUM44PE	Loss	3	GAA>CA	E28fsX16	Truncating	0.4	−	wt	+
SUM185PE	Loss	5	CAG>TAG	Q144X	Truncating	0	−	wt	+
SUM52PE	Loss	6	CGA>TGA	R213X	Truncating	0.3	−	wt	+
SUM190PE	Loss	9	CAG>TAG	Q317X	Truncating	0.4	±	nd	nd

Source: [36, 46, 52], +: normal expression, −: not detectable, nd: not determined, wt: wild type, ±: barely detectable, ++: overexpression.