Promoter Methylation Status of Breast Cancer Susceptibility Gene 1 and 17 Beta Hydroxysteroid Dehydrogenase Type 1 Gene in Sporadic Breast Cancer Patients

Table 1

Primers used for PCR amplification after HpaII digestion, along with the target band size and annealing temperatures.

Gene	Primers sequence ()	Annealing temperature	Amplicon size	Reference
BRCA1	F: TTGGGAGGGGGCTCGGGCAT		500 bp	[32]
	R: CAGAGCTGGCAGCGGACGGT
17βHSD-1	F: AGACCATCCTCACCAACAGG		238 bp	[32]
	R: CCTGGCCCTGTCATTTTTAG