Semaphorin 3A Increases FAK Phosphorylation at Focal Adhesions to Modulate MDA-MB-231 Cell Migration and Spreading on Different Substratum Concentrations

Figure 2

Sema3A regulates cell speed, but not persistence, to alter motility dynamics on collagen and fibronectin, but not laminin. ((a)–(c)) Rose plots showing the migration trajectories of 10 representative tracks for collagen (a), fibronectin (b), and laminin (c) on substrata coated with low, intermediate, and high concentrations of ECM. Scale bar = 10 μm. Average cell speed and directional persistence were measured for collagen ((d) and (e)), fibronectin ((f) and (g)), and laminin ((h) and (i)). Sema3A enhanced motility at lower coating concentrations of both collagen and fibronectin but inhibited motility at higher coating concentrations of collagen and fibronectin. Interestingly, Sema3A inhibited motility on at intermediate coating concentrations of laminin. Graphed data represent the mean ± SEM from a minimum of 30 cells from four independent experiments. , , and indicate statistical difference relative to IgG1 Fc control; two-sample -test.

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