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International Journal of Biodiversity
Volume 2014 (2014), Article ID 308398, 8 pages
Research Article

Genetic Diversity Assessment and Identification of New Sour Cherry Genotypes Using Intersimple Sequence Repeat Markers

1Department of Horticultural Sciences, Tarbiat Modares University (TMU), P.O. Box 14115-336, Tehran, Iran
2Horticultural Section, Stone Fruit Research Group, Seed and Plant Improvement Research Institute of Karaj (SPII), P.O. Box 31585-4119, Karaj, Iran
3Genomics Section, Agricultural Biotechnology Research Institute of Iran (ABRII), P.O. Box 85135-487, Isfahan, Iran

Received 19 January 2014; Accepted 2 February 2014; Published 10 March 2014

Academic Editor: Alexandre Sebbenn

Copyright © 2014 Roghayeh Najafzadeh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Iran is one of the chief origins of subgenus Cerasus germplasm. In this study, the genetic variation of new Iranian sour cherries (which had such superior growth characteristics and fruit quality as to be considered for the introduction of new cultivars) was investigated and identified using 23 intersimple sequence repeat (ISSR) markers. Results indicated a high level of polymorphism of the genotypes based on these markers. According to these results, primers tested in this study specially ISSR-4, ISSR-6, ISSR-13, ISSR-14, ISSR-16, and ISSR-19 produced good and various levels of amplifications which can be effectively used in genetic studies of the sour cherry. The genetic similarity among genotypes showed a high diversity among the genotypes. Cluster analysis separated improved cultivars from promising Iranian genotypes, and the PCoA supported the cluster analysis results. Since the Iranian genotypes were superior to the improved cultivars and were separated from them in most groups, these genotypes can be considered as distinct genotypes for further evaluations in the framework of breeding programs and new cultivar identification in cherries. Results also confirmed that ISSR is a reliable DNA marker that can be used for exact genetic studies and in sour cherry breeding programs.