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International Journal of Biodiversity
Volume 2014, Article ID 457309, 6 pages
http://dx.doi.org/10.1155/2014/457309
Research Article

Genetic Diversity of Parkia biglobosa from Different Agroecological Zones of Nigeria Using RAPD Markers

1Department of Cell Biology and Genetics, University of Lagos, Akoka, Nigeria
2Department of Botany, University of Ibadan, Ibadan, Nigeria
3Institute of Agricultural Research and Training, Moor Plantation, Ibadan, Nigeria

Received 13 August 2014; Accepted 16 October 2014; Published 9 November 2014

Academic Editor: Alexandre Sebbenn

Copyright © 2014 Oluwafemi Amusa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Parkia biglobosa (Jacq.) is an important leguminous tree crop in the African Savannahs useful to the natives where it is found, for domestic use. Previous diversity studies on this tree crop had been majorly on morphological and biochemical analysis. In order to capture the maximum diversity not obtained by previous research, the study aimed at evaluating the genetic diversity of accessions of this crop in the different agroecological zones in Nigeria using RAPD markers. A total of 81 scorable bands with an average of 8.1 bands per primer were amplified among the accessions studied. Intrazonal genetic diversity analysis showed a percentage polymorphism with a range of 11.11% to 65.43% among the agroecological zones studied. Although, gene diversity was highest within Humid forest agroecological zone, a low genetic distance and high genetic similarity between the agroecological zones were observed. Cluster analysis indicated six main groups of which four groups had single accessions while the two groups clustered the remaining accessions, indicating a narrowed genetic base from the 23 accessions studied.